Submitted to: Annals of Biomedical Engineering
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/1/2002
Publication Date: 9/1/2002
Citation: HENTZEN, E., MCDONOUGH, D., MCINTIRE, L., SMITH, W.C., GOLDSMITH, H.L. HYDRODYNAMIC SHEAR AND TETHERING THROUGH E-SELECTIN SIGNALS PHOSPHORYLATION OF P38 MAP KINASE AND ADHESION OF HUMAN NEUTROPHILS. Annals of Biomedical Engineers. 2002. Interpretive Summary: This paper discusses experiments on the mechanisms by which one type of white blood cell (called neutrophils) adheres to blood vessel walls. We have shown in earlier work that nutritionally vulnerable individuals have altered functions of these cells.
Technical Abstract: Recently, we reported that tethering and rolling of neutrophils in shear flow over a substrate of E-selectin signals activation of beta 2-integrins and firm adhesion via an intracellular signaling pathway involving phosphorylation of p38 MAP kinase. In the current study the objective was to examine the molecular mechanisms and shear dependence underlying activation and adhesion of beta 2-integrin during shear-induced collisions between human neutrophils and murine B cells (300.19) transfected to express either E-selectin or L-selectin. Three separate parameters of cell activation were assessed over the time course of application of a defined shear field to heterotypic cell suspensions in a cone-plate viscometer. These were the two-body collision doublet lifetime and capture efficiency, surface upregulation of CD11b/CD18, and tyrosine phosphorylation of p38 MAP kinase. The data indicate that neutrophil adhesion to E-selectin expressing 300.19 cells occurs with a fourfold higher efficiency of firm adhesion than do collisions with L-selectin or parent control cells. Visual analysis of aggregation in a transparent cone-plate rheoscope revealed that the lifetime and efficiency of doublet formation increased fourfold as the applied shear stress increased. Neutrophil tethering via E-selectin was associated with rapid activation as indicated by upregulation of surface CD11b/CD18 and phosphorylation of p38 MAP kinase within seconds of application of shear. Activation greatly exceeded that observed for neutrophils sheared alone or with B cells expressing L-selectin. A distinct dependence of activation on the magnitude of the shear rate suggests a coupling between the fluid mechanical effects of shear and signaling of neutrophil adhesion.