Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2003
Publication Date: 7/10/2003
Citation: Seal, B.S. 2003. Nucleotide and predicted amino acid sequence analysis of the fusion protein and hemagglutinin-neuraminidase protein genes among recent newcastle disease virus (ndv) isolates. phylogenetic ...attachment glycoprotein sequences.. American Society for Virology Meeting. Interpretive Summary:
Technical Abstract: Avian paramyxovirus type 1 (APMV-1), commonly referred to as Newcastle disease virus (NDV), can infect all orders of birds examined. The virus is a member of the order Mononegalovirales in the family Paramyxoviridae which is divided into two subfamilies, the Paramyxovirinae and the Pneumovirinae. During 1993 the International Committee on the Taxonomy of Viruses rearranged the order of the paramyxoviruses and placed NDV within the Rubulavirus genus, while more recently NDV has been designated an Avulavirus. The F protein gene was 1,792 nucleotides in length with a single ORF beginning with the AUG at residue 47 and ending at position 1705 followed by the UGA stop codon. Position of the stop codon for the F protein gene was invariant among all isolates. The HN protein gene was 2,002 nucleotides in length and most isolates had a single ORF beginning at position 92 that terminated at residue 1804 followed by a UAG stop codon. These viruses were represented by the highly virulent NDV isolates that had diverse geographic origins. Strains such as B1, LaSota, VGGA, TexasGB, BeaudetteC, Roakin, Kimber and an isolate from a Dove in Italy had an ORF from residues 92 to 1822, while isolates QV4, Ulster and D26 had an ORF from residues 92 to 1939 for the HN gene. The predicted sequence of the F protein was 553 amino acids in length for all NDV isolates with the first thirty residues being the most variable. As the ORF analysis revealed, the predicted amino acid sequence for the HN protein varied in length depending on the NDV isolate. Recent NDV isolates determined to be intermediately or highly virulent with diverse geographic origins had an HN protein of 571 predicted amino acids in length compared to the longer HN proteins from low virulence strains. Among all isolates cysteine residues and glycosylation sites were conserved. Phylogenetic analyses based on the HN of NDV isolates and other Paramyxovirinae members demonstrate that the avian paramyxoviruses segregate from other paramyxovirus types. NDV from California obtained during 2002 were most similar to viruses isolated from northern Mexico during 1996 and 2000.