|Cox, Nelson - Nac|
Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2003
Publication Date: 8/10/2003
Citation: Cosby, D.E., Bailey, J.S., Cox Jr, N.A., Richardson, L.J. 2003. Detection of campylobacter spp from chicken rinse with electrochemiluminescence. Poultry Science Association Meeting Abstract.
Technical Abstract: Processed chicken carcasses were purchased from local grocery stores and sampled for Campylobacter using the FSIS cultural procedures or by the IGEN PATHIGEN(TM) Campylobacter electrochemiluminescence procedure. Three replicates of 15 birds each were sampled by each method to compare the two methods. Briefly the PATHIGEN(TM) Campylobacter test uses a sandwich immunoassay format in which a polyclonal antibody specific for Campylobacter binds the organism to a paramagnetic micro particle and a second polyclonal antibody specific for Campylobacter is labeled with a compound, which becomes excited at the surface of an electrode and emits light when Campylobacter is present. The PATHIGEN(TM) Campylobacter takes about two hours to run after an enrichment process of 44 to 48 hours as compared to the standard FSIS method, which requires four days for a positive identification of Campylobacter. The PATHIGEN(TM) Campylobacter assay found 12 out of 15 positive in Rep 1 compared to 8 out of 15 positive for the FSIS method. In Rep 2, the PATHIGEN(TM) Campylobacter assay found 14 out of 15 positive compared to 12 out of 15 for FSIS. And in Rep 3, the PATHIGEN(TM) Campylobacter assay found 15 out of 15 positive, compared to 15 out 15 for the FSIS method. The agreement for each rep was 66.7%, 85.7%, and 100% for the three Replications, respectively. The overall agreement was 85.4%, with no false negatives detected with PATHIGEN(TM) Campylobacter and a total of six false positives (as compared to standard cultural procedures) over the three trials. The PATHIGEN(TM) Campylobacter assay is easy to run and detected Campylobacter on six samples that the conventional procedure missed. This system is an effective alternative to the tedious standard cultural procedure.