Skip to main content
ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #145327
Title: FIRST REPORT OF RASPBERRY BUSHY DWARF VIRUS FROM CHINA

Author
item CHAMBERLAIN, C - BLM
item Kraus, Jennifer
item KOHNEN, P - WHITMAN COLLEGE
item Finn, Chad
item Martin, Robert

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/12/2003
Publication Date: 4/1/2003
Citation: Chamberlain, C.J., Kraus, J.E., Kohen, P.D., Finn, C.E., Martin, R.R. First Report Of Raspberry Bushy Dwarf Virus From China.

Interpretive Summary: Rubus plants collected in The People's Republic of China and added to the National Germplasm Collection Repository at Corvallis Oregon were tested for viruses. One plant was found to be infected with Raspberry bushy dwarf virus (RBDV). Based on sequence analysis it was shown that this represents a new strain of RBDV. It was much more divergent from the type strain of RBDV than the resistance breaking strain of the virus that has been reported in Europe. This new strain can also be distinguished from other strains using monoclonal antibodies. The pathogenicity of this new strain of the virus is unknown, but care should be taken to ensure that the new strain of the virus is not distributed with germplasm that is distributed from the collection.

Technical Abstract: In recent years, several Rubus germplasm collection trips have been taken to The People¿s Republic of China to increase the diversity of Rubus in the USDA-ARS National Clonal Germplasm Repository, (Corvallis, OR). Before planting in the field, seedlings were tested for the presence of RBDV, Tomato ringspot virus and Tobacco streak virus by Triple Antibody Sandwich-ELISA (TAS-ELISA). One plant of R. multibracteatus H. Lev. & Vaniot (PI 618457 in USDA-ARS GRIN database), from Guizhou province in China, tested positive for RBDV. RNA was extracted from leaves of R. multibracteatus and subjected to reverse transcription-polymerase chain reaction (RT-PCR) and then sequenced. The predicted amino acid sequences were 91.6% (251/274) identical to the isolate from black raspberry. This confirms that the isolate from China is not a greenhouse contaminant but is indeed a unique strain of RBDV. There are only 5 amino acid differences between the resistance breaking and black raspberry strains of RBDV. In addition, monoclonal antibodies (McAb) to RBDV were tested against the isolate from China. In these tests, McAb D1 did not detect the Chinese isolate, whereas McAb R2 and R5 were able to detect the strain. This is the first strain of RBDV that has been clearly differentiated by McAb in standard TAS-ELISA tests. Although RBDV is common in commercial Rubus worldwide, this is the first report of RBDV in R. multibracteatus, and the first report of RBDV from China. The effects of this new strain of RBDV could be more or less severe, or have a different host range than the previously studied strains. It is more divergent from the type isolate than any other strain that has been studied to date.