Submitted to: In Vitro Cellular and Developmental Biology - Plants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/24/2003
Publication Date: 5/1/2004
Citation: Osuna, P., Barrow, J.R. 2004. The regeneration of black grama Bouteloua eriopoda Torr. Torr) plants via somatic embryogenesis. In Vitro Cellular and Developmental Biology - Plants. 40(3):299-302.
Interpretive Summary: Native black grama plants are continuously colonized by endophytic fungi. Fungi cannot be removed by conventional disinfestation methods. Healthy black grama plants were regenerated from meristematic cells surface disinfested embryonic shoots. Plants were grown to maturity and will be used to study plant-fungus interactions and to develop DNA libraries of black grama and associated key fungal endophytes.
Technical Abstract: Seeds of Bouteloua eropida, (Torr.) Torr. were surface disinfested and germinated on a carbon mineral rich medium. Callus was initiated from embryonic shoots excised from the roots on auxin supplemented medium. After callus multiplication, embryos were induced from callus. They developed into normal embryos and caryopses that subsequently developed into intact plants. Regenerated plants were transplanted into soil and grown in the growth chamber. The utilization of regenerated plants for plant-fungus associations is discussed.