Submitted to: Molecular Ecology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 1/29/2004
Publication Date: 6/1/2004
Citation: Richards, C.M., M. Brownson, S. Mitchell, S. Kresovich and L. Panella. 2004. Polymorphic microsatellite markers for inferring diversity in wild and domesticated sugar beet (Beta vulgaris). Molecular Ecology Notes 4:243-245. Interpretive Summary: This paper describes the development and implementation of 8 new microsatellite markers for sugar beet. This report doubles the current pool of publicly available microsatellite markers in this important agricultural species and will help in accession management and mapping of agronomic traits.
Technical Abstract: Sugar beet is an important agronomic crop that exhibits considerable genetic variation for morphological and physiological traits. Currently there is only a limited set of publicly available microsatellite markers for use in beet. The objectives of this work were to identify additional (simple sequence repeat) SSR markers from genomic libraries of sugar beet and test their efficacy in differentiating several beet accessions. A set of 8 microsatellite loci is presented. Sequences for representative alleles show several different repeat motifs ranging from simple di-nucleotide repeats to more complex compound repeats with both tetra and tri-nucleotide repeats. These 8 loci show very high heterozygosity levels (38 alleles scored) and differentiate a cultivated sugar beet (FC712), wild sea beet (Beta vulgaris var. maritima) and an accession of garden beet (Fst between accession =0.452).