Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/17/2002
Publication Date: 1/17/2002
Citation: Gao, W., Raska, D., Yu, J., Kohel, R.J., Chen, J., Stelly, D.M. 2002. Wide-cross radiation hybrids(wrhs): A tool for physical mapping of plant genomes? [abstract]. Plant, Animal, and Microbe Genomes X Conference. Paper No. P646.
Technical Abstract: Genome maps are important to genome analysis and genetic manipulation. Radiation hybrid (RH) mapping has been highly effective for high-resolution physical mapping of human and certain animal genomes, but has been little used in plants. Resolution of RH maps is often higher and regionally complementary to resolution offered by meiotic crossing-over. Herein, we describe a simple approach, wide-cross whole-genome radiation hybrid (WRH) mapping, and preliminary test results from cotton. We used the genome of one species, Gossypium barbadense, to rescue radiation-segmented chromosomes of another species, G. hirsutum. Mature pollen from G. hirsutum were gamma-irradiated and used to pollinate emasculated G. barbadense flowers to generate nonchimeric WRHs between those two species. A 5-Kr gamma-ray WRH mapping population was constructed. Tests with eight linkage group 9 micro-satellite markers revealed a marker retention frequency of 87-94%. Two SSR markers that co-segregated in reference linkage map were ordered along cotton linkage group 9 by the WRH method. The results suggest that WRH mapping method could complement traditional linkage mapping of cotton and other plant species.