Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/16/2003
Publication Date: 1/19/2003
Citation: YU, J., KOHEL, R.J., ZHANG, H., STELLEY, D.M., DONG, J., XU, Z., COVALEDA, L., ZHANG, L., GAO, W., SUN, S. INTEGRATIVE PHYSICAL AND GENETIC MAPPING OF THE COTTON GENOME (GOSSYPIUM HIRSUTUM L.). PLANT AND ANIMAL GENOME CONFERENCE PROCEEDINGS.
Technical Abstract: Whole-genome physical maps integrated with genetic maps have been proven essential to rapidly advance current genomic research in several species. To develop a whole-genome, integrated physical and genetic map for allotetraploid cultivated cottons that is widely applicable to the research community, we are working toward the map by use of three BAC and BIBAC libraries constructed from the Upland cotton (Gossypium hirsutum) genetic standard line TM-1. The feasibility, strategies, and approaches to developing the map of the AD cotton genome have been tested. Fingerprints from about 30,000 clones of TM-1 BAC/BIBAC libraries have been produced with an improved automated procedure that uses a robotic workstation, capillary sequencers, and advanced computer programs, and the fingerprints were assembled into large contigs. Molecular genetic and cytogenetic markers have been developed from the TM-1 clones for mapping of the cotton chromosomes. A permanent mapping population of 191 recombinant inbred lines (RIL-F7) was developed from a cross between TM-1 and 3-79, the genetic standard line for G. barbadense. We are physically locating approximately 1,000 DNA markers from the existing cotton genetic map to the TM-1 BAC contigs and genetically mapping about 1,000 new DNA markers isolated from the contigs by use of the RILs. The initial results showed that the cotton physical contigs representing respective subgenomic origins (A vs. D) can be readily assembled, sorted, and anchored to the cotton genetic map, thus making it feasible to develop a robust genome-wide map that integrates physical and genetic mapping information for the cultivated AD cotton.