Submitted to: Undergraduate Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2002
Publication Date: 4/15/2002
Citation: Sexauer, A.N., King, D.J., Stanton, J.B., Kommers, G.D., Seal, B.S., Brown, C.C. 2002. Immunohistochemical detection of newcastle disease virus in chickens inoculated with wild-type and infectious-clone-derived newcastle disease virus. Undergraduate Research Symposium. Interpretive Summary:
Technical Abstract: The U.S. poultry industry is a thriving economic entity, thanks in part to the exclusion of devastating diseases. Velogenic Newcastle disease virus circulates in many parts of the world and incursion into the U.S. would have serious consequences. Understanding what makes these velogenic strains of Newcastle disease virulent would help greatly in devising control methodologies. In this study, four groups of White Rock chickens were inoculated with four different viruses - E13-1 (nonpathogenic strain), NDFL+ (infectious clone made from E13-1), NDFL+HN (NDFL with virulence gene inserted), and KRC 139 (virulent strain). The birds were monitored clinically and euthanized, with tissues collected at 2, 5, 10, and 14 days post infection (dpi). Those given the virulent strain succumbed rapidly. Mild depression was noted in the NDFL+ group. Immunohistochemistry was used to follow the path of the virus throughout the body. Studies such as this will eventually help to predict which nonpathogenic viruses have the potential to evolve into virulence and result in an outbreak of Newcastle disease.