Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2002
Publication Date: 7/13/2002
Citation: King, D.J., Kommers, G.D., Seal, B.S., Brown, C.C. 2002. Biological and molecular characterization of recent newcastle disease virus iosoates before and after passage in chickens. American Veterinary Medical Association Abstract. Interpretive Summary:
Technical Abstract: Six Newcastle disease virus (NDV) isolates recovered from chickens, exotic, and wild birds were characterized before and after four passages in specific-pathogen-free 2-week-old white leghorn chickens. Hemagglutination-inhibition assays against a battery of monoclonal antibodies and nucleotide sequence analysis of the fusion protein cleavage site of the isolates were utilized to determine if antigenic or genetic changes had occurred as a result of chicken passage. The results were compared to pathotyping tests previously performed and to the Office International des Epizootes criteria for NDV virulence. Marked virulence increase was observed after passages with one of the six isolates, an isolate from an exotic dove. An isolate from an exotic pheasant was virulent before passage and demonstrated increased virulence after passage. Passage in chickens produced no marked change in virulence of the other four isolates. Although there was antigenic diversity among the isolates there was no antigenic or fusion protein cleavage site change associated with any of the observed changes in virulence of the isolates.