Author
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Kargalioglu, Yahya |
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Submitted to: Weed Science Society of America Meeting Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 2/13/2003 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Using complex nitrogen sources in mass production of Mycolepiodiscus terrestris (MT) as a biocontrol agent utilizing the liquid culture fermentation processes is a cost-effective way of producing this pathogenic fungus. The use of complex nitrogen sources such as Pharmamedia (Registered Trademark) or Solulys AST (Registered Trademark) hinders nutritional studies on the growth of MT. Identification of the specific nutritional components or concentrations necessary for maximum microsclerotia formation and microscierotia fitness as a biocherbicide require the development of a defined medium. Various sources of inorganic and organic N were examined for use in a defined medium for MT. Lysine, glutamate, arginine, aspartate, and an amino acid mixture (patterned after the AA in Pharmamedia) were used as the main N sources in the presence of glucose as the carbon source. Among the amino acids analyzed, glutamate, arginine, and aspartate were found to have the highest microsclerotia yields; and the microsclerotia produced provided excellent spore yields. However, the 24-hour germination rate for microsclerotia produced with these amino acids were observed to be lower relative to other nitrogen sources. Several inorganic N sources, ammonium sulfate [(NH4)2SO4)] showed the highest yield of microsclerotia. However, the optimum requirement of amino acids and inorganic nitrogen sources as well as C sources are still under investigation. |
