Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/29/2002
Publication Date: 1/16/2003
Citation: CEPICA, S., ROHRER, G.A., MASOPUST, M., KUBICKOVA, A., MUSILOVA, P., RUBES, J. PARTIAL CLONING, CYTOGENETIC AND LINKAGE MAPPING OF THE PORCINE RESISTIN (RSTN) GENE. ANIMAL GENETICS. 2003. v. 33. p. 381-383. Interpretive Summary: In this study a gene which is important in the regulation of fat metabolism (resistin) was mapped in the pig. The location of resistin in the pig was determined to be at chromosome 2 on the q21 band. This position is within the confidence interval for a QTL determined to affect backfat thickness and should be considered as a candidate gene for the QTL.
Technical Abstract: To determine exon-intron boundaries of the human RSTN (resistin, resistin to insulin or found in inflammatory zone 3-FIZZ3) gene the cDNA sequence was aligned with human chromosome 19 working draft DNA sequence as it has been cytogenetically mapped to 19p13.3-19p13.23. Primer pair A was designed to encompass parts of exons 2-4 and intervening introns. The PCR performed with these primers have several bands. A band with size closest to the length predicted from human DNA sequence was cut out of the gel, cloned and sequenced. The 897 bp sequence was deposited in the EMBL database (accession number AJ457069) and compared with sequences in NCBI databases using BLAST4. This sequence matched parts of exons 2, 3 and 4 of the human cDNA sequence (NM_020415) with identities 93% (41 of 44), 92% (39 of 42), and 89% (35 of 39) respectively. Deduced amino acid sequence (55 amino acids) from the amplimer was compared with GenBank CDS translations, PDB, SwissProt, PIR and PRF databases using the BLASTP function of BLAST. It showed an identity of 76% (42 of 55, e = 1e-12) with amino acid sequence translated from human resistin cDNA sequence (NM_020415). From AJ457069 primer pair B was designed yielding a 653 bp amplimer. Identity of the amplimer was confirmed by sequencing.