Skip to main content
ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #138925
Title: DIFFERENTIAL REGULATION OF PROTEIN SYNTHESIS IN SKELETAL MUSCLE AND LIVER OF NEONATAL PIGS IN EXPERIMENTALLY-INDUCED SEPSIS

Author
item KIMBALL, SCOT - PENNSYLVANIA STATE UNIV
item ORELLANA, R - BAYLOR COLLEGE OF MED
item O'CONNOR, P - BAYLOR COLLEGE OF MED
item BUSH, J - BAYLOR COLLEGE OF MED
item THIVIERGE, C - BAYLOR COLLEGE OF MED
item SURYAWAN, A - BAYLOR COLLEGE OF MED
item NGUYEN, H - BAYLOR COLLEGE OF MED
item JEFFERSON, L - PENNSYLVANIA STATE UNIV
item Davis, Teresa

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/2002
Publication Date: 4/20/2002
Citation: Kimball, S.R., Orellana, R.A., O'Connor, P.M.J., Bush, J.A., Thivierge, C., Suryawan, A., Nguyen, H.V., Jefferson, L.S., Davis, T.A. 2002. Differential regulation of protein synthesis in skeletal muscle and liver of neonatal pigs in experimentally-induced sepsis [abstract]. Federation of American Societies for Experimental Biology Conference. Part II, 16(5):A782.

Interpretive Summary: Not Necessary for an Abstract

Technical Abstract: Sepsis decreases muscle protein synthesis and increases liver protein synthesis in adults. To examine the differential responses of protein synthesis in the two tissues during sepsis, translational control mechanisms were examined in neonatal pigs treated with lipopolysaccharide (LPS). Protein synthrsis was repressed in muscle and enhanced in liver during LPS-induced sepsis. Sepsis did not alter eukaryotic initiation factor (eIF)2 phosphorylation or eIF2B activity in either tissue. Binding of eIF4G to eIF4E to form the active mRNA binding complex was repressed in muscle and enhanced in liver during sepsis. In both tissues, alterations in eIF4G binding to eIF4E were inversely correlated with eIF4E association with its binding protein, 4E-BP1. Phosphorylation of 4E-BP1 and ribosomal protein S6 kinase, S6K1, was reduced in muscle during sepsis, but increased in liver. Overall, the resuts suggest that protein synthesis in both skeletal muscle and liver is regulated during sepsis by modulation of the mRNA binding step in translation initiation. However, in muscle, the mRNA binding step is repressed whereas in liver it is enhanced.