Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/19/2002
Publication Date: 12/9/2002
Citation: WIGHT, C.P., TINKER, N.A., KIANIAN, S.F., SORRELLS, M.E., O'DONOUGHUE, L., GROH, S., SCOLES, G.J., LI, C.D., WEBSTER, F.H., RINES, H.W. A MOLECULAR MARKER MAP IN KANOTA X OGLE HEXAPLOID OAT (AVENA SP.) ENHANCED BY ADDITIONAL MARKERS AND A ROBUST FRAMEWORK. GENOME. 2002. V. 46. P. 28-47.
Interpretive Summary: A knowledge of the organization of the genes and other DNA sequence components of a crop plants' genetic material, termed a genetic map, is a valuable tool for scientists involved in gene discovery and genetic crop improvement. Development of a genetic map for the cereal oat has been difficult because oat has a larger and more complex genome, or genetic material content, than many other crop species. This study improved the previous genetic map of oat including adding more than 600 markers identifying sites along the map to the 700 previously known and incorporating new information on how the marker sites are joined together physically in the genetic material. The improved map provides geneticists and breeders a framework tool for further oat genetic map improvement, the study of gene function, and the application of identified marker sites and genes to enhancement of productivity, disease resistance, and improved seed quality traits in oat.
Technical Abstract: Molecular mapping of cultivated oats was conducted to update the previous reference map constructed using a recombinant inbred (RI) population derived from Avena byzantina C. Koch cv. 'Kanota' X A. sativa L. cv. 'Ogle.' In the current work, 607 new markers were scored, many on a larger set of RI lines (133 vs. 71) than previously reported. A robust, updated framework map was developed to resolve linkage associations among 286 markers. The remaining 880 markers were placed individually within the most likely framework interval using chi-square tests. This molecular framework incorporates and builds on previous studies including physical mapping and linkage mapping in additional oat populations. The resulting map provides a common tool for use by oat researchers concerned with structural genomics, functional genomics, and molecular breeding.