|Coe Jr, Edward|
Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2002
Publication Date: 3/14/2002
Citation: CONE, K., COE JR, E.H., GARDINER, J., MCMULLEN, M.D., FANG, Z., SCHROEDER, S., SANCHEZ-VILLEDA, H., POLACCO, M.L., DAVIS, G., YIM, Y. IMAP: THE INTEGRATED GENETIC AND PHYSICAL MAP OF MAIZE. MAIZE GENETICS CONFERENCE ABSTRACTS. 2002. p. 21. Interpretive Summary:
Technical Abstract: As a resource for future maize genomics research, we have developed the integrated genetic and physical map, which we have designated iMap. The key components of iMap are: a marker-dense high-resolution genetic map, a set of physical map segments (bacterial artificial chromosome [BAC] contigs), anchoring information that ties the genetically mapped markers to the BAC contigs, and an interactive interface for display. The genetic map was constructed using the intermated B73/Mo17 (IBM) recombinant-inbred population and a combination of marker types, including RFLPs, SSRs, MITEs, etc. The physical map is being produced by fingerprint analysis of BAC clones from libraries made using three different restriction enzymes. Together, the libraries represent over 20-fold coverage of the genome. Anchors between the genetic map and BAC contigs were made by screening the BAC libraries with RFLP core markers and with overgo probes derived from a unigene set of 10,000 ESTs. A key feature of iMap is the interactive user interface, which displays side-by-side views of the genetic and physical maps for each chromosome. The genetic map shows loci detected by multiple marker types. The physical map shows BAC contigs that are detected by genetically mapped markers. Multiple options are available for viewing the linkages between the genetic and physical maps--including locus and contig queries--and for retrieving probe data, screening images, and Genbank entries.