|El Balaa, Mohamad|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2002
Publication Date: 9/1/2002
Citation: Holowachuk, S.A., El Balaa, M.F., Buddington, R.K. 2002. The antibacterial activity of pancreatic juice: Relations with feeding state and protein secretion [abstract]. Annual Meeting of Animal Physiology Proceedings.
Interpretive Summary: None required.
Technical Abstract: The antibacterial activity (ABA) in various secretions provides innate immunity, and the bacterial overgrowth with pancreatic insufficiency suggests the ABA of pancreatic juice (PJ) controls small intestinal bacterial populations. Yet the patterns and signals of secretion for pancreatic ABA (pABA) are unknown. This study examined pABA over a 12 h period and the relations with feeding state and protein secretion using pigs with re-entrant catheters placed in the pancreatic duct and duodenum, and fed at 0800 and 1600. pABA was assayed by a microplate method that measured 24 h growth of Micrococcus pyogenes in tryptic soy broth in the presence and absence of PJ. The lag phase was consistent, but the slope of the exponential phase of M. pyogenes growth varied between samples. pABA peaked 1 h before feeding and was lowest for 1-2 h after feeding, but the magnitude of differences varied among pigs. In contrast, protein concentrations peaked at feeding, corresponding with secretion of digestive enzymes, and were lowest before feeding. Our findings indicate pABA secretion is 1)regulated independent of digestive enzymes, and 2) by different cell types or involve different regulatory signals.