Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 3/20/2002
Publication Date: 4/17/2002
Citation: Ji, L.L., Lay, D., Chung, E., Fu, Y., Parkin, K., Peterson, D.M. 2002. Effects of avenanthramides on oxidant and antioxidant status in rats. Journal of Federation of American Societies for Experimental Biology.
Technical Abstract: Avenanthramides (AVA) are a group of major non-flavonoid phenolic acids present in oat (Avena sativa L.). Recent research reveals that they have potent antioxidant function in vitro. The present study was designed to investigate the efficacy of dietary AVA supplementation on oxidant production, antioxidant defense and oxidative stress in rats at rest and after an acute bout of exercise. Forty-eight female Sprague-Dawley rats (age 6-7 wk, body wt ~60 g) were randomly assigned to two experimental groups and fed one of the following two AIN-93 based diets (average feed time 50.3 days): control (C) and supplementation with 1 g/kg AVA-Bc [N-(3',4'-dihydroxycinnamoyl)-5-hydroxyanthranilic acid]. Half of each group of rats was subjected to an acute bout of treadmill exercise at 22.5 m/min, 10% grade for 1 h and killed, whereas the other half group was killed at rest at a matching time. Final body wt did not differ between AVA (224 3 g) and C (227 3 g) rats. Ex vitro oxidant production rate, using the 2',7'-dichlorofluorescein method, was increased in the heart (p<0.05), unchanged in the liver, kidney and deep vastus lateralis muscle (DVL), and decreased in soleus muscle (p<0.05) with AVA. AVA did not alter exercise-induced oxidant production in DVL and soleus (p<0.1). Rested AVA-treated rats had higher superoxide dismutase (SOD) activity in DVL, liver and kidney (p<0.01), but lower SOD activity in the heart, compared to C rats. After exercise, AVA rats had higher SOD activity in DVL and kidney but lower in heart (p<0.05). Lipid peroxidation (malondialdehyde, MDA) was increased with exercise in heart, liver and DVL (p<0.05), and AVA lowered MDA level in the heart (p<0.05). We concluded that dietary AVA supplementation can selectively influence tissue oxidant-antioxidant balance, and this effect may be related to its ability to modulate the cellular antioxidant defense system. (Supported by USDA)