Submitted to: Workshop on Molecular Pathogenesis of Marek's Disease and Avian Immunology
Publication Type: Abstract Only
Publication Acceptance Date: 10/6/2002
Publication Date: 10/6/2002
Citation: Silva, R.F., Lupiani, B., Reddy, S.M. 2002. The effect of deleting or inverting the 132 base pair repeats in a pathogenic mdv [abstract]. Workshop on Molecular Pathogenesis of Marek's Disease and Avian Immunology. p. 49. Interpretive Summary:
Technical Abstract: Pathogenic MDVs generally have two head-to-tail copies of a 132 base pair (bp) repeat. As the virus is serially passed in cell culture, the number of copies increases from two to more than 20 copies. Concurrently, the pathogenic virus becomes attenuated. To determine the role of the 132 bp repeats in pathogenesis, we deleted all copies of the repeats from a pathogenic Md5 virus. In a second recombinant MDV, we replaced the two repeats with 9 copies of the 132 bp repeat. A third recombinant was created by replacing the two repeats with 9 copies of the 132 bp repeat, but inserted in the reverse orientation. We will discuss the effect of these mutations on RNA transcription, virus replication and in vivo pathogenicity.