Submitted to: Molecular Genetics and Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/8/1995
Publication Date: N/A
Citation: Gorman, S.W., Banasiak, D., Fairley, C., McCormick, S.M. 1996. A 610 kb yac clone harbors 7 cm of tomato (lycopersicon esculentum) dna that includes the male sterile 14 gene and a hotspot for recombination. Molecular Genetics and Genomics 251:52-59. Interpretive Summary: We used a map-based cloning method to isolate a chromosome region containing the male sterile 14 gene of tomato. Our results suggest that this 610 Kilobase region contains the ms14 gene.
Technical Abstract: Pollen development requires both sporophytic and gametophytic gene expression. We are using a map-based cloning technique to isolate sporophytic genes which, when mutant, cause pollen abortion and a male sterile (ms) phenotype in tomato (Lycopersicon esculentum). We have genetically characterized one ms locus (ms14) using RFLP analysis and identified flanking markers. High-resolution genomic physical mapping indicates that the ms14 locus is located in a \sim300 kb region. We have identified a YAC clone with an insert size of \sim610 kb that contains the ms14-linked markers, reflects the organization of the physical map and therefore most probably contains the ms14 gene. In addition, we present evidence that the relationship between physical and genetic distance in this chromosomal region changes abruptly from \sim 105-140 kb/cM to less than 24 kb/cM, and suggest that the TG393-TG104 region is a hotspot for recombination.