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ARS Home » Research » Publications at this Location » Publication #137774
Title: REGULATION OF IMMUNOGLOBULIN BINDING AND FE RECEPTOR EXPRESSION ON BOVINE NEUTROPHILS

Author
item WORKU, M - NC AG TECH U, NC
item CAMPBELL, K - NC AG TECH U, NC
item Paape, Max

Submitted to: American Dairy Science Association Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/2002
Publication Date: 9/12/2002
Citation: Worku, M., Campbell, K., Paape, M.J. 2002. Regulation of immunoglobulin binding and fe receptor expression on bovine neutrophils [abstracat]. American Dairy Science Association Abstracts.

Interpretive Summary:

Technical Abstract: A heterogeneous population of Fc receptors is recognized on bovine neutrophils (PMN). The effect of bacterial endotoxin (LPS), dexamethasone and sodium butyrate on immunoglobulin binding and Fc receptor expression on bovine PMN was analyzed by flow cytometry. Isolated bovine PMN were treated with LPS(1000, 100 or 10ng/ml), dexamethasone(0.25mg/ml, 0.15mg/ml or 0.8mg/ml) or sodium butyrate (160 uM, 80 uM or 40 uM) or maintained in buffer for 1 hour, 37C. Cells were then incubated with purified bovine IgG1, IgG2 or IgM for 30 min at 4C. Cells were then washed and incubated with fluorescein-conjugated goat anti bovine immunoglobulin antibody for 30 minutes at 4C. Endogenously bound IgG and IgM were assessed by incubating PMN with fluorescein conjugated goat antibody to bovine IgG and IgM respectively for 30 min at 4C. The percentage of fluorescent PMN and the log mean fluorescent channel were used to assess Ig binding and Fc receptor expression. Both Ig binding and Fc receptor expression for all classes of Igs' was significantly decreased by treatment with dexamethasone. Endogenously bound IgM was significantly higher in the presence of sodium butyrate when compared to untreated cells. Bacterial LPS at 100mg/ml significantly increased the expression of Fc receptors binding IgG2. Thus in bovine PMN Fc receptor gene expression can be differentially modulated for dissection of receptor regulation and function and the identification of therapeutics.