Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 4/1/2002
Publication Date: 8/10/2002
Citation: King, A.H., Jiang, Z.H., Rohrer, G.A., Gibson, J.P., Waddington, D., Archibald, A.L. 2002. Use of radiation hybrid mapping to locate candidate genes for female reproductive traits on porcine chromosome 8. 28th Animal Genetics International Conference Proceedings. Abstract E063, p. 171.
Technical Abstract: Improvement of porcine reproductive performance by traditional selective breeding programs has been limited by the low heritabilities of traits such as ovulation rate, prenatal survival and litter size. Therefore, the identification of candidate genes for use in marker-assisted selection programmes would be particularly desirable. Quantitative trait loci (QTL) for prenatal survival, litter size, teat number and ovulation rate have been mapped to chromosome 8 in earlier studies. The number of genes mapped to pig chromosome 8 and thus positional candidate genes is limited. We have developed a radiation hybrid map of pig chromosome 8, in order to determine whether specific genes map within the QTL. A skeleton RH map based on 44 markers (including 15 genes) has been established using the Cambridge-Roblin pig-hamster whole genome RH panel (http://www.ri.bbsrc.ac.uk/radhyb/) and the Carthagene software (http://www.inra.fr/bia/T/CarthaGene/). The mapped genes include the pig homologues of the Bareilly (BMPRIB) and SPARC-like 1 (SPARCL1) genes. The resulting gene map confirms the earlier observations of extensive conservation of gene order between human chromosome 4 and pig chromosome 8, but with at least one major rearrangement. AHK is supported by a BBSRC industrial CASE studentship with Sagan as the industrial sponsors.