Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 4/20/2002
Publication Date: 8/10/2002
Citation: Milan, D., Schiex, T., Yerle, M., Rink, A., Beattie, C., Alexander, L., Hawken, R., Schook, L., Hiroshi, Y., Pomp, D., Fredholm, M., Rohrer, G.A. 2002. Integration of genetic and radiation hybrid maps of the pig: the second generation IMpRH maps. 28th Animal Genetics International Conference Proceedings. Abstract D167. p. 140.
Technical Abstract: More than 4500 markers, ESTs and genes have been mapped on IMpRH radiation hybrid panel and submitted to IMpRH Server before 30 March 2002, whereas 757 markers only were mapped on the first generation map (Hawken et al, 1999). To take advantage of the different resolutions observed on the genetic and the RH maps, maps were constructed with Carthagene software using SIMULTANEOUSLY genetic (MARC USDA families) and RH (3800 markers mapped on IMpRH panel) data. For each chromosome, a framework map was produced using a step-wise locus adding strategy (SWLA). The resulting map was tested by various procedures (flips, simulated annealing ...)to try to find alternate better maps and to check if the difference of LOD with the second most likely map is at least of 3. Frequently, using checking procedures, we were able to identify maps demonstrating that the framework map proposed by the SWLA strategy was not a framework map. Framework maps were also manually improved to ensure the best possible coverage of each chromosome. Additional markers were mapped relatively to the framework maps, and comprehensive maps were established. The aim is to propose soon reliable second generation maps to assign and map additional markers and genes. Data and maps will be available on IMpRH Server (http://imprh.toulouse.inra.fr). Groups which contributes with at least 100 markers share authorship of this poster. A total of 19 groups submitted data on IMpRH Server.