Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2002
Publication Date: 8/10/2002
Citation: Cepica, S., Rohrer, G.A., Masopust, M. 2002. Gene linkage mapping of the porcine chromosome X region harbouring QTL for fat deposition. 28th Animal Genetics International Conference Proceedings. Abstract D014. p. 82. Interpretive Summary:
Technical Abstract: The QTL for backfat thickness and intramuscular fat content on SSCX is well documented in Meishan x Western breed pedigrees. The QTL has been mapped to the chromosome region between microsatellites SW2456 and SW1943. In the French pedigree with more than 1,100 F2 animals the QTL mapped at position 73-74 Kosambi cM with 95% confidence interval ranging from 65 to 85Kosambi cM. For fine mapping and comparative positional candidate cloning, alignment of human and swine gene maps is essential. Using PCR we have partially cloned and sequenced porcine orthologs of genes from HSAX and subsequently detected SNPs (PCR-RFLPs) within seven genes. These markers have been typed on the USDA-MARC backcross pedigree. Gene location on the current USDA-MARC linkage map of chromosome X follows: AMELX-8.7 cM, POU3F-74 cM, RPS4X-74 cM, FACL-80 cM, CAPN6-81 cM, PAK3-82.5 cM, and BGN-128, 0 cM. Final order of genes mapped to the USDA-MARC 2 linkage map of the chromosome X region with 95% confidence interval of the QTL contains genes in the following order: AR-74 cM, PGK-74 cM, PLP-74 cM, ZNF261-74 cM, POU3F-74 cM, RPS4X-74 cM, SERPINA7-75.5 cM, FACL-80 cM, CAPN6-81 cM, and PAK3-82.5 cM. From comparison of cytogenetic and linkage maps it is apparent that the chromosome region belongs among those with a low recombination rate. Now with more than 20 genes on the porcine linkage map there is no evidence of rearrangements in gene order between porcine and human X chromosomes.