|Coe jr, Edward|
Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract only
Publication Acceptance Date: 3/14/2002
Publication Date: 3/14/2002
Citation: GARDINER, J., CONE, K., MCMULLEN, M.D., POLACCO, M.L., DAVIS, G., SANCHEZ-VILLEDA, H., FANG, Z., SHARAPOVA, N., YIM, Y., COE JR, E.H. AN INTEGRATED MAP FOR MAIZE. MAIZE GENETICS CONFERENCE. 2002. ABSTRACT. P. 78. Interpretive Summary:
Technical Abstract: The Maize Mapping Project (MMP) is focused on developing genetic, physical, and database resources for the maize genome. A key resource being developed by the MMP is a well-integrated genetic and physical map that will expedite the identification of DNA sequences underlying key traits that have been genetically mapped. Towards this end, a high-resolution genetic map has been developed that contains over 1800 RFLP and SSR markers. HindIII Fingerprinting of 468,000 BACs from EcoRI (162 kb), MboI (165 kb), and Hind III (137 kb) libraries is at the halfway point with 8400 BAC contigs and 7500 BAC singletons. To complete the task of integrating the two maps, several complementary approaches are being taken. AFLPs and MITEs are being used on both the high-resolution genetic map and on BAC pools to link genetically mapped AFLPs and MITEs to BACs on the physical map. Additionally, mapped SSRs are also being localized to BAC contigs using BAC pools. Seven thousand seven hundred EST unigene clusters have been anchored to 165,000 BACs using 40-bp overgo sequences designed to hybridize to single- or low-copy regions. Approximately 3700 EST unigene overgoes localize to BACs contained within a single contig. A portion of these EST unigene clusters contain previously mapped SSRs that will allow BAC contigs from the physical map to be linked to the genetic map. A portion of the BAC contigs with EST unigene anchors that contain no genetic mapping information will be localized to the genetic map by the development of single nucleotide polymorphism markers and their subsequent mapping on the high-resolution genetic map. The MMP is supported by NSF DBI 9872655.