|Coe Jr, Edward|
Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/10/2002
Publication Date: 1/10/2002
Citation: GARDINER, J., CONE, K., MCMULLEN, M.D., POLACCO, M.L., DAVIS, G., YIM, Y., SODERLUND, C., WING, R., CHEN, M., COE JR, E.H. AN INTEGRATED MAP FOR MAIZE. PLANT AND ANIMAL GENOME. 2002. ABSTRACT. P. 186. Interpretive Summary:
Technical Abstract: The Maize Mapping Project (MMP) is focused on developing genetic, physical, and database resources for the maize genome. A key resource being developed by the MMP is a well-integrated genetic and physical map that will expedite the identification of DNA sequences underlying key traits that have been genetically mapped. Towards this end, a high-resolution genetic map has been developed that contains over 1800 RFLP and SSR markers. A physical map consisting of 450,000 HindIII fingerprinted BACs from EcoRI (162 kb), MboI (165 kb), and Hind III (137 kb) libraries is well underway with completion expected in early 2002. To complete the task of integrating the two maps, several complementary approaches are being taken. AFLPs are being used on both the high-resolution genetic map and on BAC pools to link genetically mapped AFLPs to BACs on the physical map. Approximately 10,000 EST unigene clusters are being anchored to 165,000 BACs using 40 bp overgo sequences designed to hybridize to single- or low-copy regions. A portion of these EST unigene clusters contain previously mapped SSRs that will allow BAC contigs from the physical map to be linked to the genetic map. A portion of the BAC contigs with EST unigene anchors that contain no genetic mapping information will be localized to the genetic map by the development of single nucleotide polymorphism markers and their subsequent mapping on the high-resolution genetic map.