Submitted to: Bergey's Manual of Systematic Bacteriology
Publication Type: Book / chapter
Publication Acceptance Date: 7/12/2002
Publication Date: 5/12/2002
Citation: STANTON, T.B., DUNCAN, S.H., FLINT, H.J. GENUS X. ROSEBURIA. AVAILABLE FROM: HTTP://WWW.SPRINGER-NY.COM/SUPPLEMENTS/0387987711/BERGEYS_ENHANCED_PAGES/INDEX.HTML BERGEY'S MANUAL OF SYSTEMIC BACTERIOLOGY, VOL. III. . Interpretive Summary:
Technical Abstract: Roseburia named in honor of Theodor Rosebury, an American microbiologist who studied and described microorganisms indigenous to humans. Slightly curved, rod-shaped cells (0.5 by 1.5 to 5 mm) occur singly and in (dividing) pairs. Non-sporulating. Gram-negative to Gram-variable staining reaction. Taxonomically grouped within cluster XIVa of the Clostridium subphylum on basis of 16S rDNA sequence. Actively motile at 37 C by means of multiple flagella inserted along the concave side and occasionally along one end of the cell. The flagella appear as a subterminal bundle when cells are examined by scanning electron microscopy or phase contrast light microscopy. Strictly anaerobic. Chemoorganotrophic. Uses the carbohydrates D-glucose, cellobiose, D-maltose, D-raffinose, sucrose, and D-xylose as carbon and energy sources. Hydrolyzes and ferments starch. Grows in anaerobically prepared media containing volatile fatty acids, yeast extract, trypticase peptone, inorganic salts, hemin, glucose, and vitamins, beneath 95% N2-5% CO2 or 100% CO2 atmosphere. Produces H2, CO2, and large amounts of butyrate from fermentation of glucose and acetate. May produce lactate, formate, and trace amounts of ethanol. Consumes acetate which may be stimulatory during growth on carbohydrates. Indigenous to mammalian intestinal tract. Catalase negative. Isolated from mouse cecal mucosa and from human feces. Uncultured bacteria with partial 16S rDNA sequences 95-97% similar to those of Roseburia have been detected in 16S rDNA analyses of swine gastrointestinal tract samples and adult human fecal samples. Two species of Roseburia have been characterized. The mol% G + C content of the DNA is 29-42% (Tm). Type species: Roseburia cecicola.