Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/6/2002
Publication Date: 1/28/2003
Citation: KATO, K.K., PALMER, R.G. GENETIC IDENTIFICATION OF A FEMALE PARTIAL-STERILE IN SOYBEAN. GENOME. 2003. V. 46. P. 128-134. Interpretive Summary: Sexual reproduction constitutes an important event in the life cycle of a plant. Much research has been on changes in the male reproductive organs because of the possibility to produce hybrid seed. The female organ is very important because the ovule carries the egg cell. After fertilization occurs, the ovule develops into the seed. A mutation in soybean was identified that is male fertile but female partial-sterile. Our objective was to study the inheritance and genetic location of this mutation. This mutation was evident only in the first generation, and subsequent generations after cross-pollination. Both male and female gametes (sex cells) are fertile in the parent plant. This observation is very unusual. This mutation was located on the molecular map. This mutation defines a new class of mutations in soybean. Comparison of normal female development with this female partial-sterile will aid in the genetic dissection of female reproductive development in soybean.
Technical Abstract: We report here the genetic identification of a female partial-sterile derived from soybean mutant L67-3483. L67-3483, which originated from cultivar Clark after X-ray irradiation, is male and female fertile. All F1 plants in reciprocal pollinations of L67-3483 with original Clark, cultivar Minsoy or cultivar BSR 101 were female partial-sterile. Partial sterility is expressed in the heterozygous condition at a single locus and upon self-pollination this locus exhibits a 1:1 segregation pattern. This locus is located on the termini of the soybean molecular linkage group D1b+W, between simple sequence markers (SSR) Satt157 and Satt266 and linked to by 5.3cM and 1.2cM, respectively. This gene transmits through both female and male gametes and there was no segregation distortion of SSR markers linked to this gene. We concluded that this female partial-sterile gene is a new mutation class, and different from the previously reported mutation classes in soybean: sporophytic mutation, gametophytic female specific mutation, and general gametophytic mutation. Restriction of recombination around the mutant gene suggested that this gene is located near or within a small inversion(s) or a chromosomal deletion(s).