Submitted to: Bergey's Manual of Systematic Bacteriology
Publication Type: Book / chapter
Publication Acceptance Date: 7/12/2002
Publication Date: 5/12/2002
Citation: CORNICK, N.A., STANTON, T.B. THE GENUS LACHNOSPIRA. AVAILABLE FROM: HTTP://WWW.SPRINGER-NY.COM/SUPPLEMENTS/0387987711/BERGEYS_ENHANCED_PAGES/INDEX.HTML BERGEY'S MANUAL OF SYSTEMIC BACTERIOLOGY, VOL. III. . Interpretive Summary:
Technical Abstract: Lachnospira named for the filamentous or "wooly" colonies formed in agar by curved or helical cells of L. multipara. Straight or slightly curved rod-shaped cells. May appear helical. Single cells measure 0.35 to 0.6 by 2.0 to 4.0 um. Cells possess Gram-positive ultrastructure. Taxonomically grouped within cluster XIVa of the Clostridium subphylum of the Gram-positive bacteria on the basis of 16S rRNA sequence. Actively motile by monotrichous or peritrichous flagella, depending on the species. Strictly anaerobic. Grows at 30-45 C. Grows in anaerobically prepared media containing rumen fluid or yeast extract, inorganic salts and pectin, or polyglacturonic acid. May also grow in a chemically defined medium. Pectinolytic. Ferments pectin, polygalacturonic acid, fructose, and cellobiose. Acetate, formate, ethanol and CO2 are the major end products from polyglacturonic acid and pectin fermentation. Small amounts of H2 may also be produced. Methanol is also produced when pectin is fermented. Succinate, butyrate, and propionate are not produced. Does not reduce nitrate. Indigenous to mammalian intestinal tract. Isolated from bovine rumen contents and from swine feces and cecal contents. Two species of Lachnospira have been characterized. The mol% G+C of the DNA is: 38-45. Type species: Lachnospira multipara.