Submitted to: Chinese Journal of Virology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 12/31/2001
Publication Date: 12/31/2001
Citation: Qin, A., Cui, Z., Lee, L.F., Fadly, A.M. 2001. Cloning and sequence of envelope gene of subgroup j avian leukosis virus. Chinese Journal of Virology. 1:54-59. Interpretive Summary: Avian leukosis subgroup J virus (ALV-J) is an economically important virus that can cause cancer-like disease and other production problems in meat-type chickens. In an attempt to develop specific diagnostics, we used molecular methods to determine the DNA sequence of a field strain of ALV-J termed ADOL-4817. The research showed that the molecular structure of this strain of ALV-J is very similar to that of other ALV-J strains. This information about molecular structure of field strains of ALV-J will help scientists in academia and industry develop better diagnostic tests and more effective control programs.
Technical Abstract: The envelope gene of ADOL-4817 strain of avian leukosis virus subgroup J (ALV-J) was amplified by polymerase chain reaction (PCR) and cloned into TA vector. The sequence analysis results showed that the envelope gene is composed of 1746 bp, 1554 bp of which could be translated into 517 amino acids for gp85 and gp37. The molecular weight of envelope protein is 57.7kDa. There are 15 potential glycosylation sites in the envelope protein, 13 of which is located in gp85. Analysis of sequences of envelope gene indicates that ADOL-4817 showed a high degree of sequence identity to other ALV-J strains, and most closely related to the envelope gene of endogenous virus EAV-HP but divergent from other ALV subgroup A-E . These data support the hypothesis that envelope gene of avian leukosis virus subgroup J maybe acquired by recombination with expressed sequences.