Submitted to: Plant Molecular Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/12/2001
Publication Date: 1/1/2002
Citation: Interpretive Summary: Plant genomics promises to accelerate genetic discoveries for plant improvements. Machine-driven technologies are ushering in gene structural and expressional data at an unprecedented rate. Potential bottlenecks in this crop improvement process are steps involving plant transformation. With few exceptions, genetic transformation is an obligatory final step by which useful traits are engineered into plants. In addition, transgenesis is most often needed to confirm gene function, following deductions made through comparative genomics, expression profiles, and mutation analysis. This article reviews the use of recombinase systems to deliver DNA more efficiently into the plant genome.
Technical Abstract: This article reviews the developments of site-specific recombinases for the manipulation of the plant genome. Emphasis is placed on methods relevant to plant transformation, particularly those that generate single copy transgenes at random or site-specific locations.