Submitted to: World Agriculture Conference
Publication Type: Abstract only
Publication Acceptance Date: 6/7/2002
Publication Date: 7/1/2002
Citation: Lim, C.E., Yildirim, M., Klesius, P.H. 2002. Effect of substitution of cottonseed meal for soybean meal on growth, hematology and immune responses of tilapia (oreochromis niloticus). World Agriculture Conference. Interpretive Summary:
Technical Abstract: A study was conducted to evaluate the effect of replacement of CSM for SBM on growth performance, hematology and immune responses of tilapia, Oreochromis niloticus. Four isonitrogenous, isocaloric diets containing 0, 19, 38 and 57% CSM as substitutes on an equal nitrogen basis for 0, 33.3, 66.7 and 100% of SBM were each fed to juvenile tilapia (5.7g average weight) in four replicate aquaria to apparent satiation twice daily for 12 weeks. Dry matter feed consumption was not affected by dietary levels of CSM but weight gain and feed efficiency were adversely affected as the dietary CSM levels increased to 38% or higher. No significant differences were observed among the weight gain and feed efficiency of fish fed diets containing 19 to 57%. Total cell count and red blood cell count were not significantly affected by increasing levels of dietary CSM. Hematocrit and hemoglobin were similar for fish fed diets without or with 19% CSM but significantly decreased when CSM levels were increased to 38% or higher. Serum protein concentration and lysozyme activity were not affected by dietary levels of CSM. Superoxide anion production measured by nitroblue tetrazolium assay significantly increased for fish fed the highest (57%) CSM diet. Phagocytic activity of macrophage of fish fed the 38% CSM diet was significantly higher than that of the control but did not differ from other treatments. Results of this study indicate that one-third of SBM in tilapia diet can be replaced by 19% CSM without affecting the growth performance, hematocrit and hemoglobin. Increasing CSM level to 38% or higher increased superoxide anion production and phagocytic activity of macrophage.