Submitted to: American Oil Chemists' Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 11/29/2001
Publication Date: N/A
Technical Abstract: High performance liquid chromatography and electrospray mass spectrometry (ESI) was used to characterize isoflavone glycosidic conjugates and minor glycosides extracted from peanut meal. The selected extraction method combined the removal of isoflavones and their conjugates with an alcoholic solvent following lipid removal with a nonpolar solvent. A reverse phase microbore high-performance column was used for the separation, and the compounds were detected with a UV detector operated at 260 nm. ESI mass spectrometry operated in the positive mode gave two major fragments for the isoflavone conjugates. The fragment ions in the spectra were the M + H ion and the protonated aglycon representing the loss of the glycoside. The 6"-O-acetylglycoside conjugates were found for daidzein, genistein, and glycitein. The mass spectra of the glycosidic conjugates extracted from peanut hearts were identical to the spectra of glycosidic conjugates isolated from soybeans and authentic standards. Other minor glycosides were also detected in the extract.