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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #134342
Title: CHARACTERIZATION OF NOVEL STARCHES FROM NEW CORN LINES

Author
item JI, Y - ISU
item WONG, K - ISU
item HASJIM, J - ISU
item Pollak, Linda
item Duvick, Susan
item JANE, J - ISU
item WHITE, P - ISU

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The objectives of this research were to evaluate functions and structures of starches from exotic x adapted inbred lines and exotic breeding crosses (exotic populations x adapted lines), and to establish relationships between the fine structure and functional properties of the starches. Several lines from forth and fifth successive generations containing a high frequency of kernels with unusual thermal properties as measured on a Differential Scanning Calorimeter were selected for further characterization. All selected starches from the developmental lines had greater peak viscosity, final viscosity and set back values as measured on a Rapid Visco Analyser (RVA) than did starch from normal corn inbreds. Significant differences were observed in starch-granule size distributions and shape distributions of the selected starches. Strong correlations were found between DSC and RVA properties and the proportion of large granules with equivalent diameter. No significant differences were observed in the apparent amylose content of starch as measured with Iodine Titration. Measurements with high-performance anion-exchange chromatography (HPAEC) revealed that all selected unusual starches had a lower normalized concentration of chains with a degree of polymerization (dp) of 15-24 and/or a greater normalized concentration of chains with a dp of 6-12. Overall, chains with a low ToG had a higher relative concentration of branch chains shorter than dp 13 than did normal starch. These results suggest that incorporation of exotic alleles into Corn Belt germplasm is an excellent means to obtain value-added traits to produce starch with desirable functions.