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Title: MUTAGENESIS AND IN VIVO STUDIES IN MAREK'S DISEASE VIRUS ENCODED INTERLEUKIN-8 LIKE GENE (VIL8)

Author
item CUI, XIAOPING - MICH STATE UNIV
item Lee, Lucy
item Gimeno, Isabel
item REED, WILLIE - MICH STATE UNIV
item Reddy, Sanjay

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/13/2002
Publication Date: 12/31/2002
Citation: Cui, X., Lee, L.F., Gimeno, I.M., Reed, W.M., Reddy, S.M. 2002. Mutagenesis and in vivo studies in marek's disease virus encoded interleukin-8 like gene (vil8)[abstract]. American Veterinary Medical Association. p. 18.

Interpretive Summary:

Technical Abstract: Marek's Disease virus (MDV) encodes a chemokine like gene designated as vIL8. This gene is located within the repeat sequences flanking the unique long region of the MDV genome. Using recA assisted restriction endonuclease cleavage method we have deleted both copies of vIL8 gene from MDV cosmid clones. We have also constructed mutants in which the "DKR" (Asp-Lys-Arg) motif in vIL8 was changed to a chemokine conserved "ELR" (Glu-Leu-Arg) motif. It is known that chemokines with an ELR motif are able to attract the migration of neutrophils (heterophils in chicken). Transfection of these mutated cosmids into cells in culture resulted in the generation of recombinant MDVs. The first recombinant has both copies of vIL8 deleted and the second has the DKR motif changed to ELR. The construction and biological characteristic of these recombinants will be discussed.