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Title: REAL TIME QUANTITATION OF VIRAL AND PROVIRAL LEVELS OF SUBGROUP J AVIAN LEUKOSIS VIRUS

Author
item PANDIRI, ARUN - MICH STATE UNIV
item LUPIANI, BLANCA - MICH STATE UNIV
item REED, WILLIE - MICH STATE UNIV
item Fadly, Aly

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/13/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: An assay for the quantitation of viral RNA and proviral DNA of subgroup J avian leukosis virus (ALV-J) was developed. Primers were designed on gp85 region of the viral envelope and detected all of the ALV-J isolates tested in this study. This assay can be used to quantitate viral RNA directly from plasma and tissue culture supernatants without the need to separate the nucleic acid, however quantitation of proviral DNA requires prior isolation of the nucleic acid. Results suggest that direct real-time quantitation of viral RNA is a quick and more accurate method that can be used for diagnosing and quantifying ALV-J viremia than traditional tissue culture based methods.