Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2001
Publication Date: 1/1/2002
Citation: Guo, B., Cao, Y., Coy, A.E., Lee, R.D., Holbrook, Jr., C.C., Lynch, R.E. 2002. Evaluation of drought tolerance and relationship with aflatoxin contamination [abstract]. Phytopathology. 92:S33. Interpretive Summary:
Technical Abstract: Corn and peanuts become contaminated with aflatoxins when subjected to prolonged periods of heat and drought stress. The effect of drought tolerance on aflatoxin contamination is not clear. The objectives of this research were to evaluate preharvest aflatoxin contamination in commercial corn hybrids known to have drought tolerance and to determine the correlation of drought tolerance with aflatoxin contamination. Gene expression under drought stress had been studied using DD-RT-PCR. Fifteen genotypes with different levels of drought tolerance were grown in two locations in Georgia in 2000 and 2001. Each location had two treatments, irrigation and not irrigation. The field evaluation on drought tolerance and aflatoxin production have demonstrated that drought tolerant commercial lines, in general, had lower aflatoxin contamination in drought condition. One poly(A)-anchored oligonucleotides and 10 arbitrary primers are used to differentiate gene expression. Polymorphic mRNA transcripts have been identified. Some cDNA fragments, up- or down-regulated by induced drought stress, have been cloned and sequenced. Further Studies are needed to characterize these cloned fragments in relationship with drought tolerance and aflatoxin formation.