Submitted to: Advances in Genome Biology and Technology
Publication Type: Abstract Only
Publication Acceptance Date: 2/6/2002
Publication Date: N/A
Technical Abstract: Expressed gene sequences (ESTs)are among the more highly conserved genome landmarks, however a robust, relatively inexpensive, high-throughput method to convert gene sequences represented as ESTs into genetic loci that can be followed in segregating populations such as those found in public plant breeding programs has been elusive. Using an EST as a labeled anchor primer, we have been following the products of PCR amplification after ligation of adaptor sequences generated with a number of restriction endonucleases. Results show that amplification and detection is possible with this approach, and that polymorphism is evident with some primer combinations. The next steps in our research will be to follow segregation, determining informative primer x restriction enzyme combinations, and estimating polymorphic information content of the generated markers.