Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2002
Publication Date: 8/11/2002
Citation: Connor, E.E., Sonstegard, T.S., Keele, J.W., Bennett, G.L., Williams, J., Papworth, R., Ashwell, M.S. Mapping of mammary gland-derived expressed sequence tags (ests) in cattle.. Animal Genetics International Conference Proceedings.
Technical Abstract: To improve the comparative map between the cattle and human genomes, bovine expressed sequence tags (ESTs) were assembled into tentative consensus (TC) sequences and those with an expression bias for the mammary gland (equal to 75% of ESTs in TC) were selected for radiation hybrid (RH) and linkage mapping. To initiate mapping, selected TC sequences were aligned with a human genome draft sequence to predict gene identity, map position, and genomic sequence structure information. Of more than 400 TC sequences analyzed, approximately 100 met criteria as candidates for mapping. These sequence alignments were used to design primer pairs for PCR that amplified a predicted intron, which limited this number to 58 primer pairs. PCR products amplified from bovine genomic DNA from sires of the USDA Meat Animal Research Center (MARC) reference population were directly sequenced to verify amplification of the targeted gene region and to identify single nucleotide polymorphisms to be used as markers for linkage mapping. Currently, 22 bovine genes are being placed on the USDA-MARC Linkage Map and/or the Roslin RH Map. Mapping of bovine ESTs improves the bovine comparative map with the human genome sequence and assists in the integration of the bovine linkage and physical maps.