Author
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Nemchinov, Lev |
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Hammond, Rosemarie |
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Submitted to: American Phytopathological Society
Publication Type: Abstract Only Publication Acceptance Date: 4/10/2002 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Beet mosaic virus (BtMV), a member of the economically important Potyvirus group, was first reported in Beta vulgaris in Germany in the late 1950s. It is distributed worldwide in all major beet growing areas. To better understand the molecular biology of the virus, we have determined the nucleotide sequence of cloned viral cDNA. BtMV has a single open reading frame typical for Potyviruses. Amino acid sequences of the CI RNA helicase, nuclear inclusion (NIa) protein, RNA-dependent RNA polymerase (RdRp), and capsid protein (CP) show significant similarities to homologous sequences from other potyviruses. Putative proteolytic cleavage sites for the NIa protease and the group-specific signature at the NIa-NIb junction were predicted by analogy to consensus sequences and genome arrangements of potyviruses. The RdRp identifiers common for positive-strand RNA viruses are also conserved in BtMV. The DAG motif, important for aphid transmission in Potyviruses, is present near the N terminus of the CP. BtMV appears to be a distinct species of genus Potyvirus with the most closely related species being Peanut Mottle Virus (~60% amino acid identity). |