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United States Department of Agriculture

Agricultural Research Service

Title: IMPROVED TOLERANCE TO ENVIRONMENTALLY INDUCED OXIDATIVE STRESSES IN TRANSGENIC TOMATO OVEREXPRESSING ASCORBATE PEROXIDASE)

Author
item Wang, Yueju
item Wisniewski, Michael
item Meilan, Richard
item Webb, Robert
item Fuchigami, Leslie

Submitted to: Abstract of International Horticultural Congress
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2002
Publication Date: 6/21/2002
Citation: WANG, Y., WISNIEWSKI, M.E., MEILAN, R., WEBB, R., FUCHIGAMI, L. IMPROVED TOLERANCE TO ENVIRONMENTALLY INDUCED OXIDATIVE STRESSES IN TRANSGENIC TOMATO OVEREXPRESSING ASCORBATE PEROXIDASE. ABSTRACT OF INTERNATIONAL HORTICULTURAL CONGRESS. 2002.

Interpretive Summary:

Technical Abstract: We studied the effect on oxidative stress resistance of overexpressing, in transgenic tomato (Lycopersicon esculentum) plants, a cytosolic ascorbate peroxidase (APX) gene derived from pea (Pisum sativum). Transformants were selected using Kanamycin resistance and confirmed by PCR, Southern and Northern analysis. Several independently-transformed lines were obtained and evaluated for resistance to oxidative stresses resulting from exposure to salinity and high-temperatue. APX enzyme activity in leaves of the transgenic plants was several-fold greater than that of the wild-type (WT) plants under non-stressed conditions. Several lines of transformants were more resistant to heat, salt, cold, and paraquat than the non-transformants. Additionally, seeds obtained from the transformed plants germinated at 9 degres C in the dark, in contrast to seeds from the WT plants which did not germinate under these conditions. Shoots of transformed lines grew in media containing 0.20 M NaCl and produced roots at 40 degrees C whereas the WT plants grew poorly in the salt medium and produced no roots at 50 degrees C. A full quantitative assessment of the resistance of the transformed lines of tomato to oxidative stress is in progress.

Last Modified: 8/24/2016
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