Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 1/1/2003
Publication Date: 1/1/2003
Citation: LOOKHART, G.L., BEAN, S., BIETZ, J.A. HPLC OF GLUTEN MONOMERIC PROTEINS. BOOK CHAPTER. 2003. Interpretive Summary: Book Chapter - no interpretive summary needed
Technical Abstract: High performance liquid chromatography (HPLC) is an analytical method that uses a liquid pumping system to accurately deliver solvents through a column or columns each packed with particles of a specific size (1.5- to 10- m) and with specific bonded phases. The end result is the ability to separate complex mixtures in minutes. HPLC is a superb tool as it is complementary and often superior to previous methods for characterization of complex cereal proteins. This chapter describes the reversed-phase and ion-exchange HPLC separations of gluten monomers. It summarizes the hundreds of reports that describe such studies; with references to more comprehensive reviews and provides protocols for these separations.