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Title: EFFECTS OF OSMOTIC STRESS AND BOVINE SERUM ALBUMIN ON SPERM MOTION CHARACTERISTICS AND PLASMA MEMBRANE INTEGRITY IN BOARS

Author
item Guthrie, Howard
item Welch, Glenn
item CRITSER, J - UNIVERSITY OF MISSOURI

Submitted to: American Society of Animal Science Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/12/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The cell volume excursion associated with exposure to osmotic stress motility loss and increased death in porcine spermatozoa. This experiment was done to determine effects of osmotic stress and bovine serum albumin (BSA) treatments on sperm viability and motion characteristic. Semen from ten boars, extended in Beltsville Thawing Solution, was incubated at 38 C for five min in phosphate-buffered saline (PBS) with or without 0.3% BSA a final osmolalities ranging from 80 to 1170 mOsmoles/kg (mOsm) and then returned to isosmotic conditions. The percent motile sperm (MOT) and measures of sperm motion were determined using a Hobson Sperm Tracker, and the proportion of sperm cells with plasma membrane integrity (PMI) was determined by flow cytometric analysis of the fluorescence of the nuclear stains SYBR-14 and propidium iodide. MOT decreased significantly (P