Submitted to: Journal of Antibiotics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/9/2002
Publication Date: 6/1/2002
Citation: TAKAMATSU, S., ZHANG, Q., SCHRADER, K., ELSOHLY, H.N., WALKER, L.A. CHARACTERIZATION OF MYCOTYPHA METABOLITES FOUND TO BE INHIBITORS OF CELL ADHESION MOLECULES. JOURNAL OF ANTIBIOTICS. 2002. V.55(6):585-592. Interpretive Summary: Extract from a type of fungus that grows in the soil was found to inhibit the action of other compounds that can cause inflammation and are involved in the spread of cancer in an organism. Three compounds were identified to be the active components of the extract and the structure of these compounds was determined.
Technical Abstract: Three inhibitors of cell adhesion based on LFA-1/ICAM-1 were isolated from the cultured broth of the fungal strain Mycotypha sp. UMF-006. These compounds were identified by spectroscopy to be cytochalasin E; 5,6-dehydro-7-hydroxy derivative of cytochalasin E; and delta(6,12)- isomer of 5,6-dehydro-7-hydroxy derivative of cytochalasin E. All of these components inhibited adhesion of HL-cells to CHO-ICAM cells at IC50 values of 30 ug/mL for cytochalasin E, 75 ug/mL for 5,6-dehydro- 7-hydroxy derivative of cytochalasin E, and 90 ug/mL for delta(6,12)- isomer of 5,6-dehydro-7-hydroxy derivative of cytochalasin E.