|Goodwin, Stephen - Steve|
Submitted to: Fungal Genetics Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/17/2002
Publication Date: N/A
Technical Abstract: Both mating type loci of Mycosphaerella graminicola were recently cloned and sequenced (WAALWIJK ET AL., FUNG. GENET & BIOL., in press). Within the non-idiomorphic sequences homology was found with palI, encoding a membrane receptor from Aspergillus nidulans, with apc, a putative component of the anaphase promoting complex from Schizosaccharomyces pombe and with a DNA -(apurinic or apyrimidinic) lyase from S. pombe. Tblastn searches against the genomic sequence of N. crassa revealed that all of these homologs as well as the mat locus reside on the same contig, #2.82. Mating type genes are among the most widely studied genes in filamentous fungi and sequences of these genes as well as their flanking regions are available from many species, particularly from ascomycetes. We therefore selected the mating type locus to perform comparative genomics among filamentous fungi and demonstrated, using recently developed bioinformatic tools, significant synteny between Fusarium spp., Leptosphaeria maculans, M. graminicola and Septoria passerinii. This shows that the genome sequence of Neurospora crassa greatly facilitates comparative genomics among ascomycetes.