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United States Department of Agriculture

Agricultural Research Service


item Wonderling, Laura
item Wallace, Frederick
item Call, Jeffrey
item Tamplin, Mark
item Feder, Ingrid
item Luchansky, John
item Pearce, Rachel
item Oser, Alan
item Palumbo, Samuel

Submitted to: Meeting Abstract
Publication Type: Abstract only
Publication Acceptance Date: 2/1/2002
Publication Date: 6/30/2002
Citation: Wonderling, L.D., Wallace, F.M., Call, J.E., Tamplin, M.L., Feder, I.E., Luchansky, J.B., Pearce, R., Oser, A., Palumbo, S. 2002. Genomic fingerprinting of salmonella recovered from swine carcass and fecalsamples at a slaughterhouse. International Associate for Food Protection. Abstract #12, p.87.

Interpretive Summary:

Technical Abstract: Previously, 100 swine carcasses were swabbed and 60 corresponding fecal samples were collected on 10 days over a 30-day period. From carcass (73%) and fecal (33%) samples that were positive for Salmonella, a total of 582 isolates were characterized by PFGE. There were 13 unique XbaI restriction profiles, with the majority (71%) of the isolates having profile types "F" (36%) or "B" (35%). While both the "F" (S. Typhimurium DT104-like PFGE profile) and "B" profiles were found on at least 8 of 10 sampling days, profile "I" was found on 3 days, profiles "A" and "D" on 2 days, and the remaining 8 profiles on only 1 of the 10 sampling days. A single isolate obtained from a carcass 18 hours post-chill during the survey period also displayed profile type "F". Examining multiple isolates from a given carcass revealed a single profile in 40 of 57 (70%) samples tested. Similarly, multiple isolates from a given fecal sample displayed a asingle profile in 16 of 19 (82%) samples tested. In general, analyses of the 15 paired carcass-fecal samples revealed that clonal types found in feces were typically found on the carcass and vice-versa, suggesting that fecal isolates from a given animal contaminated the carcass. These data suggest that although each group of animals can introduce new clonal types into the slaughterhouse, the majority of Salmonella associated with a given animal exhibit low genomic diversity.

Last Modified: 8/24/2016
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