Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/31/2001
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: Backcrossing has been used successfully to identify major genes that affect quantitative traits. Because yield is such a complex trait, using backcrossing to increase yield is likely to be successful only if loci with large effects differ between the parents. The objectives of this research were to develop high yielding BC1 and BC2 lines with U.S. cultivars as recurrent parents and exotic germplasm as donor parents and to identify SSR marker differences between these lines and the recurrent parent. Twenty BC1 and BC2 lines yielded significantly more than the recurrent parent in replicated tests at 4 locations in 2000. To identify regions that were backcrossed into the lines, comparisons were made using pairs of lines each with the same donor parent and 82 SSR loci. Comparing 3 pairs of lines, each with the same donor parent, approximately 30% of the SSR loci were polymorphic with respect to the recurrent parent. For the polymorphic loci, the pair of backcross lines had the same SSR marker allele from the donor parent 60% of the time. These loci may be linked to genes important to increasing yield and could represent diversity not present in the current U.S. gene pool.