Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2001
Publication Date: 1/1/2002
Citation: Xu, G., Guo, B., Lynch, R.E. 2002. Identification of the drought-inducible genes in peanut by MRNA display [abstract]. Mycopathologia. 155:92. Interpretive Summary:
Technical Abstract: Drought stress severely affects yield and predisposes peanut to preharvest aflatoxin contamination. Breeding for drought tolerance is one of the strategies in developing aflatoxin-tolerant peanut lines. Hence, understanding the mechanism of drought tolerance and developing molecular markers are needed for screening and characterizing peanut germplasm for drought tolerance in relation with aflatoxin contamination. There are no reports on genetic mapping of molecular markers linked to drought tolerance in peanut. The alternative approach is to minitor changes in gene expression as affected by induced water stress and to identify differentially expressed transcripts. In order to differentiate gene expression in the drought-susceptible and drought-tolerant peanut genotypes, the differential display RT-PCR has been applied to investigate gene expression pattern in response to induced drought stress. One poly(A)-anchored oligonucleotides (H-T11G) and 10 arbitrary primers were used. Various number of bands were observed with the combination of primers, ranging in size from 150 to 500bp. Polymorphic mRNA transcripts have been identified. Some cDNA fragments, up- or down-regulated by induced drought stress, have been cloned and sequenced for BLAST analysis. Eight polymorphic bands have been cloned. One clone sequence has high homology with strawberry 40S ribosomal protein s12 mRNA and the full-length cDNA has been cloned and sequenced as a putative rebosomal mRNA in peanut.