|Brown, Charles - Chuck|
Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/16/2001
Publication Date: 3/7/2002
Citation: CULLEY, D.E., EVANS, B.B., BROWN, C.R. INTROGRESSION OF THE LOW BROWNING TRAIT FROM THE WILD MEXICAN SPECIES SOLANUM HJERTINGII INTO CULTIVATED POTATO (S. TUBEROSUM L.). EUPHYTICA. 125(3):293-303. 2002. Interpretive Summary: Bruising of potatoes during harvest and handling costs potato growers and processors tens of millions of dollars each year. Many of the potato varieties currently grown in this country bruise quite easily, and the normal methods of breeding for less bruise-prone potatoes have not resulted in significant improvements. We used a wild relative of potato from Mexico that is extremely resistant to bruising to introduce this trait into cultivated potatoes. By crossing the wild relative with another potato relative, and then with cultivated potato, we were able to select plants that had a very high resistance to bruising and can now be used in standard breeding programs to produce new varieties of potato that are much less prone to bruising. These new varieties will benefit the potato industry by reducing the losses due to bruising, as well as the cost of sorting out the bruised potatoes during processing.
Technical Abstract: Internal discoloration of tubers resulting from impact damage (blackspot bruise) is a serious quality problem in potato production and utilization, reducing profits to growers and increasing costs for processors. Resistance to blackspot bruise has been identified in the wild species Solanum hjertingii and is therefore a potential germplasm resource for genetic resistance to this problem. A bridging cross between S. hjertingii and a cultivated diploid clone was used to produce a triploid hybrid population that exhibited very low tuber browning potential, indicating a dominant pattern of inheritance for this trait. The triploid progeny were subjected to in vitro chromosome doubling and the resulting hexaploid clones were screened for browning potential. A hexaploid clone selected for low browning was reciprocally crossed with cultivated S. tuberosum cultivars exhibiting high susceptibility to blackspot bruise. Tubers obtained from the seed progeny of these 4x-6x crosses (hereafter referred to as the BC1 populations) were evaluated for browning potential and polyphenol oxidase (PPO) activity. Tubers from the BC1 populations displayed a very low potential for melanin production, while PPO activity was quite variable. The low Pearson correlation coefficient (r2= 0.45), between browning potential and PPO activity suggests that the mechanism of blackspot bruise resistance derived from S. hjertingii cannot be explained simply as a reduction in the initial PPO activity. The expression of substantial resistance to browning and dominant expression pattern in these BC1 progeny indicate that utilizing genetic elements derived from S. hjertingii provides a robust approach for developing blackspot bruise resistant potato varieties.