Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 2/2/2002
Publication Date: 3/25/2002
Citation: WANG, Y., GARVIN, D.F., KOCHIAN, L.V. GENES INDUCED BY PI, K, OR FE DEFICIENCY OR NO3- RESUPPLY IN TOMATO ROOTS: ARRAY ANALYSIS REVEALS NOVEL GENES THAT MAY PLAY A ROLE IN PLANT MINERAL NUTRITION. AMERICAN SOCIETY OF PLANT BIOLOGISTS ANNUAL MEETING. 2002. Interpretive Summary:
Technical Abstract: A selection of 1280 root mineral nutrition-related cDNAs were arrayed on nylon membranes and hybridized with mRNA from tomato plants exposed either to nitrate at different time points after nitrogen was withheld for 48 hrs, or to phosphate (Pi), potassium (K), or iron (Fe) deficiencies. In addition to upregulating expression of ion transporters, nitrate and nitrate reductases, and metabolic enzymes, nitrate was found to also upregulate water channels, root Pi and K+ transporters, genes potentially involved in transcriptional regulation, stress response genes, and ribosomal protein genes. Pi, K and Fe deficiencies, on the other hand, upregulated other novel genes including MAP kinases, 14-3-3 proteins, and a suite of genes related to signaling, general stress responses and other cellular functions. There were several potentially interesting genes that were rapidly upregulated by all four treatments. These include a leucine zipper transcription factor and hemoglobin homolog, which appear to be induced specifically by these mineral stresses and not temperature or salt stresses. Additionally, a number of mineral transporters, including both families of nitrate transporters (NRT1 and NRT2), a Pi transporter, and a K+ channel were found to be inducible by all four nutrient treatments, implying some type of networking or common elements in the regulation of N, P, K and Fe nutrition. The identification of these novel mineral nutrition-related genes and our current and future studies into the function of some of these genes, are providing new avenues of research into the molecular basis for the regulation of plant mineral nutrition.