Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/20/2001
Publication Date: N/A
Technical Abstract: Microbial culture Clavibacter ALA2 transformed linoleic acid into a variety of unsaturated hydroxy fatty acids. In our previous work six novel fatty acids were identified: (1) 12,13,17-trihydroxy-9(Z)- octadecenoic acid; (2) 12-hydroxy-13,16-epoxy-9(Z)-octadecenoic acid; (3) 7,12-dihydroxy-13,16-epoxy9(Z)-octadecenoic acid; (4) 12,17;13,17-diepoxy- 9(Z)-octadecenoic acid; (5) 7-hydroxy-12,17;13,17-diepoxy-9(Z)- octadecenoic acid; and (6) 12,17;13,17-diepoxy-16-hydroxy-9(Z)- octadecenoic acid. Now, we have also identified another compound 12,13,16- trihydroxy-9(Z)-octadecenoic acid by GC/MS analysis. The optimum conditions for production of this compound was 70 hr of incubation at 30 to 35 degrees C and pH 7.0-7.5. Bioconversion pathways for tetrahydrofuranyl fatty acids and bicyclic fatty acids are different. The intermediate for the production of tetrahydrofuranyl fatty acids is 12,13,16-trihydroxy-9(Z)-octadecenoic acid and the intermediate for the production of bicyclic fatty acid is 12,13,17-trihydroxy-9(Z)- octadecenoic acid. Thus, Clavibacter ALA2 effectively oxidized linoleic acid at C-7, -12 -13, -16, and/or -17.